A method for the quantitation of conjugated bile acids in dried blood spots using electrospray ionization-mass spectrometry

K A Mills, I Mushtaq, A W Johnson, P D Whitfield, P T Clayton

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39 Citations (Scopus)

Abstract

Bile acid concentrations are elevated in the blood of neonates with cholestatic hepatobiliary disorders providing a possible means of screening for treatable conditions including biliary atresia. A method is described for the determination of concentrations of conjugated bile acids in dried blood spots using electrospray ionization mass spectrometry. Bile acids were eluted from the blood spots using methanol containing, as internal standards, the taurine and glycine conjugates of D4-chenodeoxycholic acid and D4-cholic acid. The samples were then reconstituted in acetonitrile/water and injected by autosampler into the electrospray source operating in negative ion mode. Optimal conditions were determined for both single quadrupole and tandem mass spectrometry analysis. Blood spot bile acid profiles were studied in two groups of infants ( 25 mumol/L; n = 49), and a control group (n = 96). The best discrimination between the two groups was provided by measurements of taurodihydroxycholanoates (normal
Original languageEnglish
Pages (from-to)361-8
Number of pages8
JournalPediatric Research
Volume43
Issue number3
DOIs
Publication statusPublished - Mar 1998

Keywords

  • Bile Acids and Salts
  • Biliary Atresia
  • Blood Chemical Analysis
  • Case-Control Studies
  • Cholestasis
  • Evaluation Studies as Topic
  • Humans
  • Hyperbilirubinemia
  • Infant
  • Infant, Newborn
  • Jaundice, Neonatal
  • Mass Spectrometry
  • Metabolism, Inborn Errors
  • Peroxisomal Disorders

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