A laboratory study on O-2 dynamics and photosynthesis in ice algal communities: quantification by microsensors,O-2 exchange rates, C-14 incubations and a PAM fluorometer

Ronnie Glud, S Rysgaard, M Kuhl

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Abstract

The present study compared O-2 exchange measurements, fluorometry and C-14 incubations for measuring productivity of sea-ice algae. It is demonstrated that brine leaking out of ice during freezing conditions is supersaturated with respect to O-2, while the melting water released during thawing is undersaturated with O-2, This results in a highly variable O-2 concentration at the ice-water interface during expanding or shrinking of sea ice. Consequently, great care should be taken when inferring biological activity from O-2 exchange rates, whether they are obtained via microprofiles, bulk incubations or direct measurement Of O-2 Concentrations in water below sea ice. Accounting for the O-2 dynamics related to changes in sea-ice structure, the photosynthetic activity of a mixed ice algal culture, as measured by net O-2 exchange rates and C-14 incubation, co-varied as a function of biomass and irradiance. The ratio between the net O-2 efflux and the gross C fixation rate (the photosynthetic quotient) was similar for frozen and non-frozen ice algal communities and averaged 1.43 +/- 0.48, Quantification of the relative electron transport rate (ETR) from photosystem II (PSII) and the minimum fluorescence yield (F-0) by a pulse-amplitude-modulated (PAM) fluorometer offers a fast, simple and non-invasive approach for estimating activity and biomass of ice algal communities. The relative ETR measurements correlated with the other 2 measures for primary production. However, the correlation was non-linear, leading to a poor resolution of the fluorometer approach at higher photosynthetic activities, A similar observation was made for the correlation between measurements and the concentration of chlorophyll a.
Original languageEnglish
Pages (from-to)301-311
Number of pages11
JournalAQUAT MICROB ECOL
Volume27
Issue number3
DOIs
Publication statusPublished - 2002

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Keywords

  • CHLOROPHYLL FLUORESCENCE
  • Marine & Freshwater Biology
  • MICROELECTRODES
  • EUKARYOTIC ALGAE
  • PRODUCTIVITY
  • REGIONS
  • Ecology
  • NORTHERN BALTIC-SEA
  • IN-SITU
  • Microbiology
  • PHYTOPLANKTON BLOOM
  • PHOTOINHIBITION
  • BENTHIC MICROALGAE

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