TY - JOUR
T1 - A genetic study of the NOS3 gene for ischemic stroke in a Chinese population
AU - Du, Danhua
AU - Gao, Peng
AU - Hu, Linsen
AU - Yang, Yumei
AU - Wang, Feng
AU - Ye, Lin
AU - Zhang, Xuan
AU - Chang, Ming
AU - Zhao, Jiexu
AU - Wu, Jiang
AU - Megson, Ian L
AU - Wei, Jun
PY - 2008
Y1 - 2008
N2 - We recruited 560 unrelated patients with ischemic stroke and 153 unrelated controls to undertake a genetic analysis for association between the NOS3 gene and ischemic stroke. All the subjects were Chinese of Han descent. Because the NOS3 gene spans about 21 kb of DNA and contains 26 exons, we selected a single nucleotide polymorphism (SNP) rs3918181, an A to G base change located in intron 14 of the gene, as a DNA marker. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs3918181 (RsaI site). The chi-square (chi(2)) goodness-of-fit test showed that the genotypic distributions of the marker were not deviated from Hardy-Weinberg equilibrium in both the patient group (chi(2) = 0.166, p = 0.684) and the control group (chi(2) = 0.421, p = 0.517). The cocaphase analysis showed allelic association of rs3918181 with ischemic stroke in males (chi(2) = 4.04, p = 0.044, OR = 1.43, 95% CI 1.01 approximately 2.02) and frequency of allele A was significantly higher in male patients than male control subjects. The chi(2) test revealed genotypic association between rs3918181 and ischemic stroke in males (chi(2) = 4.26, df = 1, p = 0.039, OR = 1.61, 95% CI 1.02 approximately 2.53) but not in females. The present work suggests that rs3918181 is associated with ischemic stroke in male patients. This finding gives further evidence in support of the eNOS association with ischemic stroke in the Chinese population.
AB - We recruited 560 unrelated patients with ischemic stroke and 153 unrelated controls to undertake a genetic analysis for association between the NOS3 gene and ischemic stroke. All the subjects were Chinese of Han descent. Because the NOS3 gene spans about 21 kb of DNA and contains 26 exons, we selected a single nucleotide polymorphism (SNP) rs3918181, an A to G base change located in intron 14 of the gene, as a DNA marker. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs3918181 (RsaI site). The chi-square (chi(2)) goodness-of-fit test showed that the genotypic distributions of the marker were not deviated from Hardy-Weinberg equilibrium in both the patient group (chi(2) = 0.166, p = 0.684) and the control group (chi(2) = 0.421, p = 0.517). The cocaphase analysis showed allelic association of rs3918181 with ischemic stroke in males (chi(2) = 4.04, p = 0.044, OR = 1.43, 95% CI 1.01 approximately 2.02) and frequency of allele A was significantly higher in male patients than male control subjects. The chi(2) test revealed genotypic association between rs3918181 and ischemic stroke in males (chi(2) = 4.26, df = 1, p = 0.039, OR = 1.61, 95% CI 1.02 approximately 2.53) but not in females. The present work suggests that rs3918181 is associated with ischemic stroke in male patients. This finding gives further evidence in support of the eNOS association with ischemic stroke in the Chinese population.
M3 - Article
C2 - 20428408
SN - 1178-7074
VL - 1
SP - 65
EP - 68
JO - International Journal of General Medicine
JF - International Journal of General Medicine
ER -