• UHI Institute of Health Research and Innovation, Centre for Health Science, Old Perth Road, Inverness, IV2 3JH

    United Kingdom

  • United Kingdom

  • 77 Citations
  • 5 h-Index
20142019
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Personal profile

Research expertise

My basic research interests are directed towards development of analytical based methods for the detection and quantification of diverse biomolecules in different biological matrices. The human body is exposed to several environmental pollutants and other toxicological compounds as well as endogenously produced metabolites. There are processes and in checks within the body to combat the deleterious effects of these compounds. However, when the deleterious effect overwhelms the protective processes, it leads to modification of biomolecules that may alter their functions causing disease state. My interest lies is detecting such subtle changes using a combination of analytical techniques to develop translational medicines.

Up to now my research mainly focused on detection of small molecules, novel lipid peroxidation products and bioactive lipids as well as establishment of mass spectrometry based methods for their qualitative and quantitative analysis. Three years of my experience with metabolic tracing at University of Birmingham gave me an extensive theoretical and practical knowledge about central carbon metabolism and how it is altered in disease condition such as different types of cancer. My PhD provided me gain hands on experience with different mass spectrometers such as LTQ-Orbitrap-XL ETD, QTrap 5500 and Q-TOF instruments. Working with different mass spectrometers over the years, I gained experience in different fragmentation techniques (CID, PSD, ETD) and corresponding spectra analysis. I have a strong knowledge in development of different shotgun and LC-MS data acquisition methods, such as data dependent acquisition (DDA), MSE and multiple reactions monitoring (MRM).

An important aspect of my research was to develop different kinds of MS-based approaches to detect and quantify different lipid peroxidation products in inflammatory disease models such as Acute Pancreatitis, Type 2 Diabetes and Malarial parasite samples. During my PhD work I have developed semi-targeted LCMS methods for the identification of lipid peroxidation derived carbonyls, dicarboxylates, hydroperoxides, hydroxides and isoprostanoid compounds on QTrap 5500 (ABSciex) and method validation on high resolution QTOF (Absciex 5600).  Though most of my work was focused on detection and quantification of lipid peroxidation biomarkers, I am also familiar with various aspects of protein prost-translational modifications analysis, particularly ones caused by lipid peroxidation derived carbonyls. Besides the operational and application skills, I am familiar with routine maintenance of the HPLC and nUPLC instruments which I am doing for the past years at the laboratory. 

Education/Academic qualification

Doctor of Philosophy, Aston University

3 Jan 201330 Oct 2016

Fingerprint Dive into the research topics where Alpesh Thakker is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 3 Similar Profiles
Phospholipids Chemical Compounds
Phosphatidylcholines Chemical Compounds
Mass spectrometry Chemical Compounds
NAD Medicine & Life Sciences
divinyl benzene Chemical Compounds
Isocitrate Dehydrogenase Medicine & Life Sciences
Antineutrophil Cytoplasmic Antibodies Medicine & Life Sciences
Peer Review Medicine & Life Sciences

Research Output 2014 2019

  • 77 Citations
  • 5 h-Index
  • 10 Article
7 Citations (Scopus)

Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

Teo, A. CK., Lee, S. C., Pollock, N. L., Stroud, Z., Hall, S., Thakker, A., Pitt, A. R., Dafforn, T. R., Spickett, C. M. & Roper, D. I., 12 Feb 2019, In : Scientific Reports. 9, p. 1-10 10 p., 1813.

Research output: Contribution to journalArticle

Open Access
Bacterial Proteins
Phospholipids
Membrane Proteins
Lipids
Styrene
2 Downloads (Pure)

A Targeted Mass Spectrometry Approach to detect and quantify Oxidised Phospholipids in plasma samples of Diabetic patients

Thakker, A., Spickett, C. M. & Pitt, A., 12 Sep 2019, In : BioRxiv. p. 1-20 20 p.

Research output: Contribution to journalArticle

Open Access
File
Phosphatidylcholines
Mass spectrometry
Phospholipids
Plasmas
divinyl benzene
2 Citations (Scopus)
1 Downloads (Pure)

Metabolic tracing reveals novel adaptations to skeletal muscle cell energy production pathways in response to NAD + depletion: [version 2; peer review: 2 approved] revised edition.

Oakey, L. A., Fletcher, R. S., Elhassan, Y. S., Cartwright, D. M., Doig, C. L., Garten, A., Thakker, A., Maddocks, O. D. K., Zhang, T., Tennant, D. A., Ludwig, C. & Lavery, G. G., 15 Nov 2019, In : Wellcome Open Research. 3, 147, p. 1-21 21 p.

Research output: Contribution to journalArticle

Open Access
File
Peer Review
NAD
Muscle Cells
Skeletal Muscle
Glyceraldehyde
1 Downloads (Pure)
Open Access
File
Mass spectrometry
divinyl benzene
Polystyrenes
Liquid chromatography
Bioactivity
5 Citations (Scopus)

The effects of oxygenation on ex vivo kidneys undergoing Hypothermic Machine Perfusion

Patel, K., Smith, T. B., Neil, D. AH., Thakker, A., Tsuchiya, Y., Higgs, E. B., Hodges, N. J., Ready, A. R., Nath, J. & Ludwig, C., 1 Feb 2019, In : Transplantation. 103, 2, p. 314-322 9 p.

Research output: Contribution to journalArticle

Perfusion
Kidney
Air
Metabolomics
Citric Acid Cycle