ملخص
A quantitative real-time PCR (qPCR) for detection of the neurotoxin of the Clostridium botulinum type C (BoNTC) encoding gene has been compared with a nested PCR (nPCR) and a conventional PCR (cPCR) using 2 toxigenic C. botulinum C1 reference strains and samples from bird tissues (n = 30) and sediments (n = 30) from wetlands where botulism outbreaks have been reported. A cPCR based on 16S ribosomal RNA sequences from 60 strains of Clostridium species was also developed to detect the genomic DNA of C. botulinum C in order to evaluate the presence of nontoxigenic strains. Quantitative PCR showed a similar sensitivity to nPCR (
| اللغة الأصلية | English |
|---|---|
| الصفحات (من إلى) | 942-6 |
| عدد الصفحات | 5 |
| دورية | Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
| مستوى الصوت | 23 |
| رقم الإصدار | 5 |
| المعرِّفات الرقمية للأشياء | |
| حالة النشر | Published - 2011 |